Insolubilized proteins and immunoassays utilizing them

US4253844A · kind A · utility

Assignee

• Technicon Instruments Corp. · US

Inventors

• Joseph Limet · Marchin, BE
• Cesar L. Cambiaso · Kraainem, BE
• Claude H. Moussebois · Brussels, BE
• Pierre L. Masson · Brussels, BE

Key dates

• Filing date: Jan 22, 1979
• Grant date: Mar 3, 1981
• Expiry date: Jan 22, 1999

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S436/801
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Insolublized antibodies, F(ab').sub.2 fragments thereof and protein antigens are made by coupling to a water-insoluble substrate using a bridging agent which covalently binds to sulphur atoms in antibody, fragment or protein rather than to free amino groups therein. This is preferably effected by using a chloracetyl-terminated bridging agent. Immunoassays utilizing these insolubilized reagents are described. One novel class of bridging agents comprising anhydride and monochloracetyl end groups, which is useful for making the said reagents, is also disclosed.