Medium for the separation of human blood leucocytes
US4255256A · kind A · utility
30Cited by
7References
8Claims
0Family size
Inventors
Key dates
| Filing date | Nov 23, 1979 |
| Grant date | Mar 10, 1981 |
| Priority date | — |
| Expiry date | Nov 23, 1999 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/48
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
A rapid one-step density separation method for separating and purifying whole blood into mononuclear and polymorphonuclear leucocyte and erythrocyte fractions wherein use is made of a separating medium consisting of a single aqueous solution of a high molecular weight sucrose or glucose polymer, and a water soluble metrizoate or diatrizoate compound, the density of the solution being greater than 1.095 gm/ml at room temperature and the concentration of the sucrose or glucose polymer lying in the range of 5 to 10% (based on weight/total volume of solution).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.