Fluorescent scavenger particle immunoassay
US4256834A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 9, 1979 |
| Grant date | Mar 17, 1981 |
| Priority date | — |
| Expiry date | Apr 9, 1999 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/808
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Novel immunoassays are provided employing discrete particulate reagents for determining an analyte which is a member of a specific binding pair-ligand and homologous receptor. The assay employs as a first reagent, a member of said pair bound to an insoluble particle (particle conjugate); as a second reagent, a label which is part of a signal producing system, bound to a member of said pair (signal label conjugate); and as a third reagent, a signal repressor comprising an insoluble particle, where the signal repressor is obstructed from interacting with said label of said signal label conjugate, when said signal label conjugate is bound to said particle conjugate. In performing the assay, the analyte, the reagents, and any ancillary materials are combined in an aqueous assay medium and the signal determined as compared to an assay medium having a known amount of analyte. The repressor greatly enhances the sensitivity and accuracy of the immunoassay in repressing the signal produced by labels which are not bound to the particle conjugate, thus substantially limiting the observed signal to label bound to the particle conjugate. The labels which are employed provide a signal which does…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.