Interferon production
US4262090A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 4, 1979 |
| Grant date | Apr 14, 1981 |
| Priority date | — |
| Expiry date | Jun 4, 1999 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/811
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Method for preparing interferon, mRNA for interferon, and competent recombinant DNA containing dsDNA and cDNA from mRNA coding for mammalian interferon. The method employs crossing a mutant mammalian cell which is semiconstitutive for interferon with a cell derived from the same or different mammal having wild type gene(s) for interferon and for the regulation of interferon synthesis and desirably having phenotypic properties allowing for selection of the hybrid cells. The desired hybrid clones are then induced to produce IF mRNA, wherein the amounts of mRNA for interferon are greatly enhanced over the amounts normally obtained from wild type cell strains. The mRNA is employed to produce cDNA which codes for the mammalian interferon. The single stranded cDNA is employed as a template to prepare dsDNA which is then combined with a replicon recognized by a microorganism host to provide a recombinant DNA. The microorganism host is transformed with the recombinant DNA, so as to provide a source for the interferon gene, as well as interferon.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.