DNA Joining method
US4264731A · kind A · utility
50Cited by
0References
10Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Apr 21, 1978 |
| Grant date | Apr 28, 1981 |
| Priority date | — |
| Expiry date | Apr 21, 1998 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/849
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A selected portion of DNA molecules having reactant ends which are capable of being joined in a ligase catalyzed reaction are pretreated so a to remove the 5'-terminal phosphate groups. Such a treatment reduces the frequency of joining an undersired combination and enhances the frequency of joining the desired combination.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.