High sensitivity assays for angiotensin converting enzyme
US4276378A · kind A · utility
5Cited by
2References
17Claims
0Family size
Inventors
Key dates
| Filing date | Nov 25, 1977 |
| Grant date | Jun 30, 1981 |
| Priority date | — |
| Expiry date | Nov 25, 1997 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/802
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A sensitive method for measuring angiotensin converting enzyme in clinical material such as serum, urine, and tissue biopsies. The method employs Hip His Leu as the substrate. The method exploits the finding that sulfate ion in the range of 0.75 through 1.1 M lowers the apparent Km for Hip His Leu from about 2.6.times.10.sup.-3 M to about 1.8.times.10.sup.-4 M. This 14-fold decrease in Km effectively permits a more sensitive assay with Hip His Leu. The assay may be fluorimetric or radioactive means to measure the presence of eznymatic reaction.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.