Support for the determination of enzyme activities and process
US4277561A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 16, 1977 |
| Grant date | Jul 7, 1981 |
| Priority date | — |
| Expiry date | Feb 16, 1997 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/826
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A support and method for the determination of enzyme activity in a biological extract is provided wherein the support comprises a fibrous material impregnated with a substrate and a water-soluble pH stabilizer comprising a first reagent suitable for a pH of enzyme activity over 7 or a second reagent suitable for a pH of enzyme activity of 7 or less; the viscose fibers are sufficiently loose in texture so as to be accessible both to products in solution and to tissuey and cellular mash or whole cells and sufficiently fine to permit adsorption and uniform distribution of reagents over the area thereof. The method comprises: PA1 (a) preparing a biological extract of the enzyme to be determined; PA1 (b) depositing a sample thereof on the support; PA1 (c) incubating said sample; and PA1 (d) placing on the support a developing reagent comprising a wetting agent and a buffer, and a coloring agent whereby the reactions are brought to a fixed pH, said pH being optimal for the colorimetric reaction between the substrate and the coloring agent.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.