Method and composition for determining an oxidized pyridine co-enzyme

US4278760A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Karl Wulff · Weilheim, DE
• Fritz Stahler
• Gerhard Michal · Tutzing, DE

Key dates

• Filing date: Jul 19, 1979
• Grant date: Jul 14, 1981
• Expiry date: Jul 19, 1999

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/008
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method for the quantitative determination of an oxidized pyridine co-enzyme which comprises reacting a sample containing oxidized pyridine co-enzyme in the presence of its specific alcohol dehydrogenase with an aliphatic alcohol of 8 to 16 carbon atoms, oxidizing the aldehyde formed with reduced flavine mononucleotide and oxygen in the presence of luciferase and of the particular FMN-reductase which is independent of the co-enzyme to be determined and of the reduced substrate co-enzyme of the latter, and measuring the resulting light emission as a measure of the initial oxidized pyridine co-enzyme content.