Method for the quantitative determination of plasminogen activators
US4278762A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Sep 11, 1979 |
| Grant date | Jul 14, 1981 |
| Priority date | — |
| Expiry date | Sep 11, 1999 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/976
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a method for the quantitative determination of enzymes in human and mammal body fluids and tissue extracts, using a substrate which has the structure EQU R.sup.1 --X--Y--Z--NH--R.sup.2 wherein X represents a group having the formula ##STR1## in which R.sup.3 is a straight or branched alkyl radical having 1 to 7 carbon atoms or a cyclohexyl or cyclohexylmethyl radical, Y represents a seryl group or a group having the formula --NH--(CH.sub.2).sub.n --CO-- in which n is an integer from 1 to 7, Z represents an arginyl or lysyl group, R.sup.1 represents hydrogen or an acyl or sulfonyl group and R.sup.2 represents an aromatic hydrocarbon radical which optionally may carry substituents. The method includes measuring by photometric, spectrophotometric or fluorescence-photometric methods the quantity of the split product NH.sub.2 R.sup.2 formed by the hydrolytic action of the biologically active factors on the substrate.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.