Diagnostic agents for the detection of proteolytic enzymes

US4278763A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Dieter Berger · Viernheim, DE
• Franz-Josef Braun · Rimbach, DE
• Werner Guthlein · Mannheim, DE
• Manfred Kuhr
• Wolfgang Werner · Hersbruck, DE

Key dates

• Filing date: Nov 30, 1979
• Grant date: Jul 14, 1981
• Expiry date: Nov 30, 1999

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2334/50
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention provides a diagnostic agent for the detection of proteolytic enzymes, comprising an absorbent carrier, a film layer, a powder mixture, a lyophilisate, a solution or a reagent tablet, containing at least one chromogen and an appropriate buffer substance, wherein the chromogen used is an indoxyl- and/or thioindoxyl-amino acid ester and/or peptide ester of the general formula: ##STR1## wherein R.sub.1, R.sub.2, R.sub.3 and R.sub.4, which can be the same or different, are hydrogen or halogen atoms, lower alkyl, lower alkoxy, aryl, aralkyl, aralkoxy, hydroxyl, carboxy, carboxy lower alkoxy, aralkoxycarbonyl, aralkyloxycarbonyl lower alkoxy, nitro or lower acylamino radicals, whereby two adjacent substituents can also represent an optionally halogenated fused benzene ring, X is a sulphur atom or an imino group optionally substituted by a lower alkyl, aryl, aralkyl or acyl radical, A is an amino acid or peptide residue and B is a nitrogen protective group conventional in peptide chemistry or derived therefrom. The present invention also provides new compounds of the above-given general formula and processes for the preparation thereof. Furthermore, the present invent…