Automated method for cell volume determination
US4284355A · kind A · utility
37Cited by
4References
6Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 29, 1979 |
| Grant date | Aug 18, 1981 |
| Priority date | — |
| Expiry date | Oct 29, 1999 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N15/1459
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Fluorescence volume exclusion signals are utilized to evaluate cell volume. Cells are suspended in a medium which is furnished with a fluorescent dye which neither penetrates nor adheres to the cells. Cells are analyzed by an optical flow cytometer, with illumination which causes the dye medium to fluoresce. Passage of a cell through the sensing zone therefore reduces the amount of fluorescent medium being illuminated, and proportionally reduces the fluorescence output signal. Fluorescence volume exclusion pulse height and area are related to cell volume.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.