Method for producing a vaccine against bacterial infections caused by pseudomonas aeruginosa
US4285936A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 10, 1979 |
| Grant date | Aug 25, 1981 |
| Priority date | — |
| Expiry date | Dec 10, 1999 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K39/104
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method for isolating a non toxic, high molecular weight polysaccharide igen from the crude slime of a Pseudomonas aeruginosa culture, and a method for inducing immunity in a host to said live organisms is described. The slime is initially prepared for the isolation procedure by separating the bacterial cells from the slime and dissolving the slime in a phosphate buffer solution. Dissolved contaminating nucleic acids are then precipitated and separated from solution. A lipid A portion of the contaminating lipopolysaccharide constituent is then removed and precipitated by acetic acid hydrolysis, and the remaining lipids present are extracted with chloroform. Nearly all of the residual nucleic acids are then removed by digestion with nucleases, and the remaining protein extracted with phenol. The aqueous and phenol layers are then separated, and the aqueous layer applied to a gel filter to isolate the antigen by column chromatography. The antigen appears in the void volume. A vaccine containing the polysaccharide antigen of this invention has been successfully tested and found to be non toxic and highly effective in inducing an immune response to the organism in a host.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.