Method and reagent for the determination of creatine kinase

US4286057A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Karl Wulff · Weilheim, DE
• Fritz Stahler
• Wolfgang Gruber · Bischofswerda, DE

Key dates

• Filing date: Feb 28, 1980
• Grant date: Aug 25, 1981
• Expiry date: Feb 28, 2000

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/81
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Creatine kinase is determined by the reaction of creatine phosphate with adenosine diphosphate with the formation of adenosine triphosphate, reaction of the latter with luciferin and oxygen in the presence of luciferase and diadenosine pentaphosphate with the formation of oxyluciferin and adenosine monophosphate, and measurement of the light thereupon emitted, the reaction being performed with a saturated concentration of adenosine diphosphate and substrate, in the presence of 1 to 10 millimoles per liter of AMP at pH values of 5.8 to 7.5, with at least 50 units of luciferase per test. It is desirable to operate in the presence of diadenosine pentaphosphate or sodium fluoride and a sequestering agent.