Diagnostic composition for the detection of leukocytes and chromogens useful therein

US4296202A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Dieter Berger · Viernheim, DE
• Franz-Josef Braun · Rimbach, DE
• Gunter Frey · Ellerstadt, DE
• Werner Guthlein · Mannheim, DE
• Manfred Kuhr

Key dates

• Filing date: Aug 2, 1979
• Grant date: Oct 20, 1981
• Expiry date: Aug 2, 1999

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/5094
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Novel diagnostic composition for the detection of leukocytes in body fluids, comprising an absorbent carrier impregnated with a buffer substance and a chromgen, wherein the chromogen is an azo dyestuff ester of the formula EQU A--N.dbd.N--B(OR).sub.n wherein PA0 A is a five- or six-membered heterocyclic radical of up to 2 hetero ring atoms selected from nitrogen, sulfur and oxygen, and wherein the heterocyclic ring may be substituted with at least one member of the group selected from halogen, lower alkyl and lower alkoxy; or PA0 A is phenyl substituted by at least one member of the group selected from lower alkyl, lower alkoxy, nitro, sulphanato and acylamino; PA0 B is benzene, naphthalene or quinolone, optionally substituted with one or two radicals selected from sulphanato, lower alkoxy and lower alkoxypoly-alkyleneoxy; PA0 R is a carboxylic acid residue or an amino acid or peptide residue having a nitrogen protective group; and PA0 n is 1 or 2.