Diagnostic reagent
US4298685A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 3, 1979 |
| Grant date | Nov 3, 1981 |
| Priority date | — |
| Expiry date | May 3, 1999 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/808
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A sample, e.g. serum, containing an antigen, hapten or other biological substance is mixed with antibodies raised against that substance which have been tagged with biotin, and with a known amount of that substance labelled with an enzyme. After the competitive complexation of the antibody with the labelled substance and the substance to be detected, avidin, immobilised on an inert support is added. The avidin binds to the biotin and causes the complex to be precipitated. The solid and liquid phases are separated by centrifugation and the amount of biological substance in the original sample is determined by measuring the activity of the enzyme in either phase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.