Spectrofluorometer
US4299486A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 5, 1980 |
| Grant date | Nov 10, 1981 |
| Priority date | — |
| Expiry date | Feb 5, 2000 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N21/6445
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
In order to provide a spectrofluorometer which measures the emission polarization spectrum of a sample at high precision and with ease, a first spectrum signal I.sub.HV (.lambda..sub.EM) and a second spectrum signal I.sub.HH (.lambda..sub.EM) are formed by scanning the wavelength of an emission monochromator. A third spectrum signal I.sub.VH (.lambda..sub.EM) and a fourth spectrum signal I.sub.VV (.lambda..sub.EM) are also formed. In these terms, the first suffix H of the signals means that excitation light irradiated at the sample has a horizontal polarization direction while the first suffix V of the signals means that excitation light irradiated at the sample has a vertical polarization direction. The second suffix H of the signals means that fluorescence light detected by the detector has a horizontal polarization direction. The second suffix V of the signal means that fluorescence light detected by the detector has a vertical polarization direction. Using these signals the spectrofluorometer produces a signal G(.lambda..sub.EM) representative of the ratio between the signal I.sub.HV (.lambda..sub.EM) and the signal I.sub.HH (.lambda..sub.EM). Also, a correction signal G(.lambd…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.