Method for determining the concentration of an L-amino acid in fermentation
US4299669A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 27, 1979 |
| Grant date | Nov 10, 1981 |
| Priority date | — |
| Expiry date | Mar 27, 1999 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/817
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Electrochemical method for determining the concentration of a carbon source and L-amino acid in a fermentation medium or cultured broth by contacting a microbial electrode consisting of an oxygen-sensitive electrode or a carbon dioxide gas-sensitive electrode combined with fixed microbial cells of a micro-organism capable of assimilating said carbon source or decarboxylating said L-amino acid with a sample solution and sensing electrically the rate of current decrease which is caused by the consumed oxygen in the solution in proportion to the concentration of carbon source; or the electric motive force which is caused by the liberation of carbon dioxide in the sample solution in proportion to the concentration of L-amino acid. Microbial electrodes and systems useful in carrying out the aforementioned method.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.