Identification and sorting of plant heterokaryons
US4300310A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jul 28, 1980 |
| Grant date | Nov 17, 1981 |
| Priority date | — |
| Expiry date | Jul 28, 2000 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA01H1/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
To label heterokaryons formed by fusion of different somatic tissue cells, somatic tissue cells from one plant are immersed in a first solution including polysaccharidase and fluorescein isothiocyanate and somatic tissue cells from a different plant are immersed in a second solution including polysaccharidase and rhodamine isothiocyanate for a period of from 12 to 18 hours, after which protoplasts emerge, with those from one plant fluorescing green-yellow and those from the other fluorescing orange-red. The protoplasts are then fused in a polyethylene glycol solution. The heterokaryons are identified and sorted by a commercial cell sorter which relies upon the fluorescence from both markers.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.