Transfer and detection of nucleic acids
US4302204A · kind A · utility
124Cited by
3References
12Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jul 2, 1979 |
| Grant date | Nov 24, 1981 |
| Priority date | — |
| Expiry date | Jul 2, 1999 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/805
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Improvements in the transfer and detection of separated nucleic acids, both RNA and DNA, are provided. For analysis of large DNA, the molecular weight segregated fractions of DNA are depurinated and fragmented to provide fractions having less than about 2 kb as single strands. With both RNA and DNA, the nucleic acid fractions are transferred after resolution to a chemically treated substrate and covalently affixed to the substrate. The resulting nucleotides affixed to the substrate are hybridized with labeled nucleotide probes and a volume exclusion agent, particularly a water soluble ionic polymer.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.