Patent · US Expired

Reagent and method for the determination of peroxidase

US4302537A · kind A · utility

8Cited by

2References

20Claims

0Family size

Assignee

Boehringer Mannheim GmbH · DE

Inventors

Karl-Dietrich Gundermann · Clausthal-Zellerfeld, DE
Karl Wulff · Weilheim, DE
Fritz Staehler · Tutzing, DE
Hans-Ralf Linke · Raisting, DE

Key dates

Filing date	Feb 11, 1980
Grant date	Nov 24, 1981
Priority date	—
Expiry date	Feb 11, 2000

Classification

Technology area (CPC Y)Emerging Cross-Sectional Technologies
CPC primaryY10S435/968
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

The present invention provides a method for the determination of peroxidase in the presence of a compound, the enzyme-catalysed oxidation of which with a peroxy compound results in the emission of light, which is measured, wherein 7-dimethylaminonaphthalene-1,2-dicarboxylic acid hydrazide of the formula ##STR1## is used as light-emitting compound. The present invention also provides a reagent for the determination of peroxidase, comprising 7-dimethylaminonaphthalene-1,2-dicarboxylic acid hydrazide, a system providing hydrogen peroxide and a buffer substance of pH 6 to 9.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.