Patent · US Expired

Alpha-amylase assay and substrates for use therein

US4304854A · kind A · utility

7Cited by
2References
14Claims
0Family size

Assignee

Inventors

Key dates

Filing dateJul 1, 1980
Grant dateDec 8, 1981
Priority date
Expiry dateJul 1, 2000

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q1/48
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

A maltodextrin phosphorylase limit dextrin in the presence of maltodextrin phosphorylase and inorganic phosphate, is used as a substrate for alpha-amylase, which initiates a series of enzymatic reactions resulting in a chromogen response which can be used to measure the concentration of alpha-amylase in a body fluid. A novel limit dextrin and its preparation also are described.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.