Agglutination assay
US4305721A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Apr 7, 1980 |
| Grant date | Dec 15, 1981 |
| Priority date | — |
| Expiry date | Apr 7, 2000 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/817
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An analyte is determined quantitatively by an agglutination assay by use of a method which includes the use of at least two analyte standards having different analyte concentrations; an analyte supported on a particulate support; specific binder for the analyte and buffered diluent for making serial dilutions of an analyte sample. The assay is preferably conducted on a test card which includes a sample portion and a standard portion, with the sample portion having a series of separated marked dilution areas for receiving dilutions of an analyte sample and the standard portion including separated marked standard areas for receiving analyte standard of different analyte concentration. By addition of binder and particles sensitized with analyte to the serial dilutions and standards, analyte can be determined quantitatively by multiplying the lowest standard concentration at which there is no visible agglutination by the highest reciprocal dilution of test sample which similarly shows no visible agglutination.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.