Immunoassay using ascitic fluid
US4307190A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 25, 1979 |
| Grant date | Dec 22, 1981 |
| Priority date | — |
| Expiry date | Oct 25, 1999 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/821
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A liquid, particularly but not exclusively a liquid of human origin such as blood serum or urine, is immunoassayed for antibodies, antigens or antibody: antigen complexes, using as a reagent in the analysis an active fraction from mouse ascitic fluid. This active fraction is a euglobulin and has the ability, like human C1q, to combine with antibody:antigen complexes but not with free antibody or antigen. Unlike human C1q, however, it remains active at high pH's and its activity is not destroyed by 0.1 M putrescine or 0.1 M hydrazine, so analyses on human body fluids can be carried out at high pH's or in the presence of putrescine and hydrazine, without interference from endogenous human C1q. The active fraction is a very broadly applicable reagent in immunoassays and particularly useful in techniques involving agglutination of latex particles.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.