Cell-free synthesis of deacetoxycephalosporin C
US4307192A · kind A · utility
15Cited by
3References
9Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jan 7, 1981 |
| Grant date | Dec 22, 1981 |
| Priority date | — |
| Expiry date | Jan 7, 2001 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/926
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A cell-free process for converting isopenicillin N, .delta.-(L-.alpha.-aminoadipyl)-L-cysteinyl-D-valine (hereinafter "LLD") and 6-substituted derivatives thereof to deacetoxycephalosporin C (DACPC) by the following reaction sequence: ##STR1## is disclosed. In addition to the starting material, the reaction system includes ATP and a fresh extract of Cephalosporium acremonium prepared and used in a manner to preserve the racemase agent or agents necessary for conversion of the isopenicillin N to penicillin N, a necessary intermediate step in the process.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.