Radioimmunological determination of procollagen (type III) and procollagen peptide (type III)

US4312853A · kind A · utility

Assignee

• Max-Planck-Gesellschaft · DE

Inventor

• Rupert Timpl · Gauting, DE

Key dates

• Filing date: Apr 13, 1979
• Grant date: Jan 26, 1982
• Expiry date: Apr 13, 1999

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S530/842
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

For the radioimmunological determination of procollagen (type III) and procollagen peptide (type III) a certain amount of radioactively tagged procollagen (type III) or procollagen peptide (type III) and a highly specific anti-procollagen (type III) serum or anti-procollagen peptide (type III) serum are brought to reaction together with a sample of an unknown content of procollagen (type III) or procollagen peptide (type III), the formed antigen-antibody complex is separated, desirably by the addition of a second antibody against the highly specific antiserum, and the radioactivity of the complex or of the supernatant liquid is measured. Highly purified procollagen peptide (type III) suitable for this purpose is obtained by the degradation of tissue or body fluids with collagenase and purification by immune adsorption and chromatography.