Immunoradiometric assay employing terminal radionuclide labeling and synthesis of conjugates for such assay

US4320109A · kind A · utility

Assignee

• University of Southern California · US

Inventors

• Walter Wolf · Backnang, DE
• Robert Nakamura · Lomita, US
• Ata Gokce · İstanbul, TR
• Manuel Tubis · Laguna Hills, US
• Timothy J. O'Brien · St. Francis, US

Key dates

• Filing date: Jun 29, 1979
• Grant date: Mar 16, 1982
• Expiry date: Jun 29, 1999

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/60
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

An immunoradiometric assay in which an antigen is reacted to its specific antibody, which complex is then separated and further reacted with a second, universal antibody. This second, universal antibody contains a chelating moiety, such as transferrin, capable of binding specifically and strongly short-lived radionuclides. The second complex, once formed is reacted with a radionuclide and the radio-activity is measured, determining thereby the quantity of the unknown antigen originally present.