Patent · US Expired

Vertical gel slab electrophoresis apparatus and method therefor

US4325796A · kind A · utility

25Cited by
3References
8Claims
0Family size

Assignee

Inventors

Key dates

Filing dateJan 30, 1981
Grant dateApr 20, 1982
Priority date
Expiry dateJan 30, 2001

Classification

  • Technology area (CPC G)Physics
  • CPC primaryG01N27/44756
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

A vertical gel slab electrophoresis apparatus includes a pair of sample units (12, 13) each of which is a pair of clamped together, spaced glass plates (23a, 23b and 24a, 24b) for containing the gel (21, 22). A casting stand is provided which holds the pair of sample units vertical and seals their bottom slots (26, 27) to prevent leakage of the gel. After polymerization, an upper buffer solution container (33) is locked on to the pair of sample units, the casting stand removed, and the remaining U-shaped structure placed in a tank (11) where electrophoresis may be initiated. A specialized seal is provided between the sample units and the upper buffer chamber (33) in the form of a resilient gasket (36, 37) stretched over the one end of a pair of sample plates and this is then wedged into a mated truncated conical sleeve extending from the bottom (34) of the upper buffer container to provide a seal which effectively isolates the upper buffer solution (32) from the lower buffer solution (28).

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.