Fermentation process for producing higher plant cells
US4326034A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 8, 1979 |
| Grant date | Apr 20, 1982 |
| Priority date | — |
| Expiry date | Aug 8, 1999 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/818
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Higher plant cells (e.g. cells of Spermatophyta) capable of growing in the presence of light and in the absence of a carbohydrate carbon source are produced from higher plant cells which require a carbohydrate source by cultivation in an aqueous medium in the presence of light, O.sub.2 and CO.sub.2. The quantity of carbohydrate is reduced during the cultivation while the concentration of dissolved oxygen is maintained at a value below 250 n mol of O.sub.2 per ml of medium. Preferably cultivation is in continuous culture and the preferred dissolved O.sub.2 concentration is from 25 to 85 n mol per ml. The CO.sub.2 present and the quantum flux density may be increased as the carbohydrate is reduced.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.