Enzyme-aminoglycoside conjugates
US4328311A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 28, 1980 |
| Grant date | May 4, 1982 |
| Priority date | — |
| Expiry date | Feb 28, 2000 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/806
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods and compositions are provided for conjugating a wide variety of compounds, particularly polyfunctional compounds, having a mercapto group, either naturally present or synthetically introduced, to a polyamino compound, particularly a polypeptide (including proteins). The method employs a haloalkyl-carbonyl compound, which is conjugated to one or more of the amino groups under mild acylating conditions. This is followed by combining the acylated polyamino compound with a mercapto containing compound, whereby the halogen is displaced by the sulfur of the mercapto group to form a stable thioether linkage. The resulting conjugates, depending on the compounds involved, can find uses in immunoassays, as hapten-antigen conjugates for the production of antibodies, and as ligand analog enzyme conjugates for use as reagents in controlling the distribution of substitution of a mercapto compound to a polyamino compound.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.