Method of preparing high-activity, low-bacteria, urease enzyme

US4330626A · kind A · utility

Assignee

• The Enzyme Center, Inc. · US

Inventors

• Henry Blair · Boston, US
• Stanley E. Charm · Boston, US
• Richard P. Crowley · Westborough, US

Key dates

• Filing date: Mar 19, 1980
• Grant date: May 18, 1982
• Expiry date: Mar 19, 2000

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/80
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

In a method of preparing the enzyme, urease, from jack beans, wherein the jack beans are reduced to a fine particle size and the fine particles so reduced constitute urease, the fine jack-bean particles thereafter irradiated for a period of time, the irradiated jack beans then recovered and employed as a source of urease enzyme, the improvement which comprises: irradiating the whole jack beans prior to any size reduction of the seeds of the jack beans, to provide a urease derived from the white seeds of the irradiated whole jack beans having an improved activity level.