.beta.-Galactosyl-umbelliferone-labeled protein and polypeptide conjugates
US4331590A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 6, 1980 |
| Grant date | May 25, 1982 |
| Priority date | — |
| Expiry date | May 6, 2000 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/806
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An improved specific binding assay method and reagent for determining a ligand in a liquid medium employing, as an enzyme-cleavable substrate label, a residue having the formula: EQU G-D-R wherein G is a glycone, D is a dye indicator moiety, and R is a linking group through which the label residue is covalently bound to a binding component of a conventional binding assay system, such as the ligand, an analog thereof, or a specific binding partner thereof. The monitored characteristic of the label is the release of a detectable product, usually a fluorogen or chromogen, upon enzymatic cleavage of the glycosidic linkage between the glycone and the dye indicator moiety. The assay method may follow a homogeneous or heterogeneous format. The preferred glycone is a .beta.-galactosyl group and the preferred dye indicator moiety is an umbelliferone residue. The improved assay is particularly suited to the determination of haptens, such as drugs, and antigenic proteins and polypeptides, including antibodies, following a homogeneous competitive binding assay format.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.