Easily split substrates for the quantification of proteases

US4335204A · kind A · utility

Assignee

• Kabi AB · SE

Inventors

• Karl G. Claeson
• Leif E. Aurell · Särö, SE
• Leif R. Simonsson · Göteborg, SE
• Salo Arielly · Kungsbacka, SE

Key dates

• Filing date: Mar 25, 1981
• Grant date: Jun 15, 1982
• Expiry date: Mar 25, 2001

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S530/802
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Easily split enzyme substrates for the quantification of proteases having the general formula EQU R.sub.1 --p-Glu--A.sub.1 --A.sub.2 --NH--R.sub.2, where PA1 R.sub.1 =H or a protective group, preferably t-butyloxycarbonyl, benzyloxycarbonyl; PA1 A.sub.1 =Gly, Ala, Val, Leu, Ile, Ser, Thr, Pro, Pip, Phe or Tyr; PA1 A.sub.2 =Arg or Lys; PA1 R.sub.2 =an aromatic, possibly substituted, hydrocarbon group, wherein --NH--R.sub.2 is a physico-chemically determinable group, preferably a chromogenic or fluorogenic group which is split by a present enzyme and then forms a cleavage product of the formula H.sub.2 N--R.sub.2 the amount of which can be quantified. Processes for the production of said substrates. Method in the laboratory diagnostics of proteases using said substrates.