Fused gene and method of making and using same

US4336336A · kind A · utility

Assignee

• President and Fellows of Harvard College · US

Inventors

• Thomas J. Silhavy · Montgomery, US
• Howard A. Shuman · Larchmont, US
• Jon Beckwith · Cambridge, US
• Maxime Schwartz · Paris, FR

Key dates

• Filing date: Jan 12, 1979
• Grant date: Jun 22, 1982
• Expiry date: Jan 12, 1999

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/62
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This invention is a method for fusing, within a bacterial host, a gene for a cytoplasmic protein to a gene for a non-cytoplasmic protein, so that the hybrid protein produced is transported to, near, or beyond the cell surface for ease of collection and purification, or for immunological use. The invention also encompasses the fused gene created by the disclosed method.