Method for selective determination of conjugated and unconjugated bilirubin
US4338095A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jul 14, 1980 |
| Grant date | Jul 6, 1982 |
| Priority date | — |
| Expiry date | Jul 14, 2000 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/146666
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method is described for the selective determination of the conjugated bilirubin (B.sub.c) or unconjugated bilirubin (B.sub.u), as well as the total bilirubin (B.sub.T), content of an aqueous liquid. The method comprises contacting the aqueous liquid with an analytical element having a dry reagent zone comprising an interactive mordant for bilirubin and thereafter detecting the absorption or emission spectra of the aqueous liquid at two or more wavelengths. The respective molar absorption or emission characteristics of B.sub.u and B.sub.c are predetermined for at least two of these wavelengths using a molecular weight of 584 for B.sub.u and a molecular weight in the range from about 750 to about 940 for B.sub.c. The B.sub.u, B.sub.c and/or B.sub.T content is determined from such spectra and absorption or emission characteristics. The interactive mordant for bilirubin is used to enhance the absorption characteristics or produce fluorescent emission of mordanted bilirubin.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.