Reagent for the determination of lipase and process for preparing same

US4343897A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Ulrich Neumann · Peißenberg, DE
• Karl-Wolfgang Knitsch · Andechs, DE
• Joachim Ziegenhorn · Starnberg, DE
• Albert Roder · Seeshaupt, DE
• Werner Zwez · Seeshaupt, DE
• Werner Kramer · Schwandorf, DE

Key dates

• Filing date: Jan 28, 1980
• Grant date: Aug 10, 1982
• Expiry date: Jan 28, 2000

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/107497
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A reconstitutable dry reagent for the turbidimetric determination of lipase, which reagent forms an emulsion upon adding water and comprises substrate oil, protective colloid, emulsifier and activator; and the process for making said dry reagent comprising preparing an aqueous emulsion of a triglyceride containing bile acid salt, colipase, at least 10% by weight of protective colloid, and at least a part of a preserving agent, lyophilizing the emulsion, mixing the lyophilizate with a buffer substance and urea, and recovering said dry reagent.