Process for separating and recovering coproporphyrin and uroporphyrin from a culture broth containing them
US4347184A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 25, 1981 |
| Grant date | Aug 31, 1982 |
| Priority date | — |
| Expiry date | Mar 25, 2001 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/83
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process for separating and recovering coproporphyrin and uroporphyrin from a culture broth containing them, which comprises PA0 (1) adjusting the pH of the liquid phase of a cultured broth containing coproporphyrin and uroporphyrin to a range of 2.5 to 4 to form a solid precipitate containing coproporphyrin and uroporphyrin and collecting the precipitate, PA0 (2) preparing an aqueous alkaline solution of the solid precipitate obtained in step (1), adjusting the pH of the solution to a range of more than 4 but not over 6, to form a solid precipitate containing coproporphyrin, and collecting the precipitate, and PA0 (3) adjusting the pH of the residue left after collection of the precipitate in step (2) to a range of 1 to 4 to form a solid precipitate containing uroporphyrin and collecting the precipitate. The active porphyrin components thus obtained have physiological activity in regulating various organisms of the body, such as, for example, improving the function of the liver.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.