Method for preparing a recombinant DNA phage
US4348477A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 22, 1979 |
| Grant date | Sep 7, 1982 |
| Priority date | — |
| Expiry date | Oct 22, 1999 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for preparing a novel recombinant DNA, which comprises (1) cleaving with an endonuclease a phage DNA having an endonuclease-sensitive region not in the DNA segment participating in temperate phage DNA replication and integration of DNA into a host chromosome but in other DNA segments, a temperate phage DNA having an endonuclease-sensitive region in the DNA segment carrying genetic information for the production of coat protein, and a DNA carrying the intended genetic information, (2) mixing together all fragments produced by said cleaving, (3) adding DNA ligase to the mixture, and (4) recovering from the resulting mixture a phage DNA having its coat protein producing ability deleted by the replacement of the DNA segment carrying genetic information for coat protein production with a DNA fragment carrying the intended genetic information.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.