Method for preparation of a recombinant DNA phage
US4348478A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 22, 1979 |
| Grant date | Sep 7, 1982 |
| Priority date | — |
| Expiry date | Oct 22, 1999 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for the preparation of a novel recombinant DNA, which comprises (1) cleaving with an endonuclease a temperate phage DNA having an endonuclease-sensitive region not in the DNA segment participating in the replication of phage DNA and the integration of phage DNA into a host chromosome but at least in the DNA segment carrying genetic information for the coat protein production and another DNA carrying intended genetic information, (2) adding DNA-ligase to the mixture of both cleft DNA's, and (3) recovering from the mixture a phage DNA having its coat protein producing ability deleted by the replacement of the DNA segments carrying genetic information for the coat protein production with a DNA fragment carrying the intended information.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.