Test for glucosylated hemoglobin and other glucosylated proteins
US4349352A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 14, 1980 |
| Grant date | Sep 14, 1982 |
| Priority date | — |
| Expiry date | Aug 14, 2000 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/723
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention is a process for a rapid and precise colorimetric method for determination of the total glucosylated blood proteins. The method is especially adapted and useful as a clinical, diagnostic aid for identification, management and treatment for diabetics. The method essentially comprises use of phenylhydrazine derivatives as reagents to determine the extent of glucosylation of hemoglobin and other proteins in the blood samples tested. The adduct of the aldehyde sugar (glucose) and the hemoglobin possesses the ketoamine structure. This structure is readily detected colorimetrically after treatment with a phenylhydrazine to give the corresponding phenylhydrazone. Phenylhydrazine and 2,4-dinitrophenylhydrazine are preferred reagents for the diagnostic process.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.