Method of and reagents for quantitative analysis of cyclic nucleotides
US4350761A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | May 15, 1980 |
| Grant date | Sep 21, 1982 |
| Priority date | — |
| Expiry date | May 15, 2000 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of quantitative analysis of cyclic nucleotides which comprises: acylating a cyclic nucleotide in an assay sample by using an acylating agent; causing said acyl-cyclic nucleotide and a specific quantity of an enzyme-labeled cyclic nucleotide formed by bonding a cyclic nucleotide corresponding to the acyl-cyclic nucleotide and an enzyme through a dicarboxylic acid to undergo competitive reaction with a specific quantity of an antibody with respect to a corresponding cyclic nucleotide; separating the enzyme-labeled cyclic nucleotide which has become bonded to said antibody and the enzyme-labeled cyclic nucleotide which has not become bonded thereto; and determining the quantity of the cyclic nucleotide by measuring the enzyme activity of either of the enzyme-labeled cyclic nucleotides. For this analysis, a reagent comprising an enzyme-labeled cyclic nucleotide is used.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.