Microbiological synthesis of beta endorphin

US4350764A · kind A · utility

Assignee

• The Regents of the University of California · US

Inventors

• John D. Baxter · West Melbourne, US
• Ivy Fettes · San Francisco, US
• John Shine · Fawn Drive, AU

Key dates

• Filing date: Mar 10, 1980
• Grant date: Sep 21, 1982
• Expiry date: Mar 10, 2000

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/849
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

DNA comprising the naturally occurring nucleotide sequence coding for amino acids 44-90 of .beta.-lipotropin and including the entire coding region for .beta.-endorphin with the exception of the C-terminal glutamine was modified, transferred to an expression transfer vector, and expressed as a fusion protein. The fusion protein was further modified in vitro to yield mature .beta.-endorphin. .beta.-endorphin was purified from a bacterial lysate. The structure and biological activity of the resulting product was proven by immunological assay, and by two independent assays designed to demonstrate biological activity.