Rapid, quantitative peptide synthesis using mixed anhydrides

US4351762A · kind A · utility

Assignee

• Bioresearch, Inc. · US

Inventors

• Michael S. Verlander · Del Mar, US
• William D. Fuller · Goleta, US
• Murray Goodman · Village of La Jolla, US

Key dates

• Filing date: Mar 10, 1981
• Grant date: Sep 28, 1982
• Expiry date: Mar 10, 2001

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K5/101
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

In the synthesis of a polypeptide chain wherein an N.sup..alpha. -urethane-protected amino acid mixed anhydride is coupled with an amino-unprotected second amino acid, the improvement comprising conducting said coupling at room temperature or above. This method provides rapid, essentially quantitative reactions without the necessity for reduced temperatures. The method is particularly useful in the solid-phase synthesis of peptides where shortened coupling cycles and improved yields of substantially pure products are obtained.