Patent · US Expired

Method for single nucleotide alteration

US4351901A · kind A · utility

15Cited by
2References
18Claims
0Family size

Assignee

Inventor

Key dates

Filing dateMar 24, 1980
Grant dateSep 28, 1982
Priority date—
Expiry dateMar 24, 2000

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12N15/102
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

A method for altering a single nucleotide at a predetermined position in a gene. The method involves the isolation of a single strand gene fragment extending up to the position before the nucleotide to be altered. A ribonucleotide or a protected deoxyribonucleotide corresponding to the desired altered nucleotide is attached at the end of this fragment. The fragment is then annealed to a complementary template which extends beyond the end of the fragment. The fragment is then extended complementary to the remainder of the template. The resulting partially mismatched double-stranded DNA is used to produce a pure DNA gene containing an altered deoxyribonucleotide at the single desired position.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.