Synthetic gene coding for influenza hemagglutinin
US4357421A · kind A · utility
11Cited by
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20Claims
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Key dates
| Filing date | Mar 24, 1980 |
| Grant date | Nov 2, 1982 |
| Priority date | — |
| Expiry date | Mar 24, 2000 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2760/16122
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A process for the production of a synthetic gene for an influenza haemagglutinin which comprises: PA0 (a) subjecting isolated vRNA to the action of reverse transcriptase to produce a double-stranded RNA/DNA hybrid; PA0 (b) digesting the RNA component of the hybrid; PA0 (c) treating the residual DNA component of the hybrid to produce a double-stranded DNA molecule having one "hairpin" end; and PA0 (d) treating the "hairpin" structure with a single-strand specific nuclease to produce a bimolecular double-stranded copy of the vRNA.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.