Creatine kinase isoenzyme electrophoretic technique and improved creatine kinase isoenzyme reagent for use therein
US4360413A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jul 20, 1981 |
| Grant date | Nov 23, 1982 |
| Priority date | — |
| Expiry date | Jul 20, 2001 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44747
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A creatine kinase reagent of the type comprising a buffer, creatine phosphate, adenosine diphosphate, D-glucose, hexokinase, glucose-6-phosphate dehydrogenase, a thiol compound, magnesium ion, adenosine monophosphate, and a coenzyme selected from a group consisting of nicotinamide adenine dinucleotide, nicotinamide adenine dinucleotide phosphate, and mixtures thereof. The reagent is characterized in that it further comprises an effective amount of an enzyme selected from a group consisting of phosphogluconate dehydrogenase and phosphogluconate dehydrogenase (decarboxylating). An electrophoretic technique for assaying the relative distribution of creatine kinase characterized in that the above reagent is employed therein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.