Fluidic assembly for an ultra-high-speed chromosome flow sorter
US4361400A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 26, 1980 |
| Grant date | Nov 30, 1982 |
| Priority date | — |
| Expiry date | Nov 26, 2000 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S209/939
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A fluidic assembly for an ultra-high-speed chromosome flow sorter using a fluid drive system, a nozzle with an orifice having a small ratio of length to diameter, and mechanism for vibrating the nozzle along its axis at high frequencies. The orifice is provided with a sharp edge at its inlet, and a conical section at its outlet for a transition from a short cylindrical aperture of small length to diameter ratio to free space. Sample and sheath fluids in separate low pressure reservoirs are transferred into separate high pressure buffer reservoirs through a valve arrangement which first permit the fluids to be loaded into the buffer reservoirs under low pressure. Once loaded, the buffer reservoirs are subjected to high pressure and valves are operated to permit the buffer reservoirs to be emptied through the nozzle under high pressure. A sensor and decision logic is positioned at the exit of the nozzle, and a charging pulse is applied to the jet when a particle reaches a position further downstream where the droplets are formed. In order to adjust the timing of charge pulses, the distance between the sensing station at the outlet of the nozzle and the droplet breakoff point is deter…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.