Process for the preparation of defective mutants of microorganisms

US4362815A · kind A · utility

Assignee

• Henkel Kommanditgesellschaft auf Aktien · DE

Inventors

• Frank Hill · Mettmann, DE
• Joachim Schindler
• Rolf Schmid · Gelterkinden, CH
• Wolfgang Preuss · Monheim, DE
• Alfred Struve · Hilden, DE

Key dates

• Filing date: Mar 7, 1980
• Grant date: Dec 7, 1982
• Expiry date: Mar 7, 2000

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P33/005
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

For the production of 17-C-steroid-.alpha.-propionic acid compounds by microbial side chain degradation on 17-C-side chain steroid substrates wild strain microorganisms selected in predetermined manner are subjected to a multi-stage mutation and selection. The resultant mutant strains are capable of supplying the desired compounds, e.g. .DELTA.-4 BNC and/or .DELTA.-1,4 BNC in high yields even in the absence of inhibitors arresting the growth and/or degradation of the steroid ring. Wild strains and mutants particularly suitable, and their choice, production and use, are described.