Novel radioassay procedure
US4372941A · kind A · utility
Inventor
Key dates
| Filing date | Sep 5, 1980 |
| Grant date | Feb 8, 1983 |
| Priority date | — |
| Expiry date | Sep 5, 2000 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/37
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A rapid, efficient and accurate method is provided for quantitatively assaying for an enzyme, such as angiotensin converting enzyme, an aminopeptidase, a carboxypeptidase, or trypsin, which catalyzes hydrolysis of a radiolabelled substrate to produce a radiolabelled remnant product preferentially soluble in a water-immiscible organic solvent, whereby the assay is conducted in a vial having a volume of not more than 20 milliliters, preferably 7 ml. or less, by incubating substrate in buffer and sample, stopping the reaction after a predetermined time with, e.g., acid or base, adding the water-immiscible organic solvent containing scintillant, capping the vial, mixing by inverting the vial whereupon a spontaneous phase separation occurs. The radioactivity of the organic phase is then counted in an appropriate scintillation counter and the enzymic activity is thereupon calculated.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.