Method for the purification of cholesterol oxidase
US4374930A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 19, 1981 |
| Grant date | Feb 22, 1983 |
| Priority date | — |
| Expiry date | Oct 19, 2001 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/815
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for the purification and recovery of cholesterol oxidase is disclosed. Cholesterol oxidase is purified by a method including the steps of: PA0 (a) contacting a solution of the cholesterol oxidase to be purified with a chromatography composition comprising an unbound water-insoluble steroid which is a nonsubstrate for cholesterol oxidase so as to adsorb the cholesterol oxidase on the steroid; PA0 (b) separating the solution from the steroid; and PA0 (c) contacting the steroid with an eluting solution containing a surfactant at a concentration between 0.001 percent and 10 percent so as to recover the adsorbed cholesterol oxidase. In a preferred embodiment, the solution containing the cholesterol oxidase to be purified is a microorganism growth medium having cholesterol oxidase activity and, prior to the step of contacting the solution with a steroid, the solution is contacted with an ion-exchange chromatography material. In yet another embodiment, the eluting solution containing the surfactant also contains a metal salt of a concentration of at least about 0.005 molar. There is also disclosed a method for forming a packed bed of a composition comprising the unbound steroid w…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.