Biological extracts and method for making same
US4376819A · kind A · utility
11Cited by
10References
8Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Feb 23, 1981 |
| Grant date | Mar 15, 1983 |
| Priority date | — |
| Expiry date | Feb 23, 2001 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/967
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A p-nitrophenyl hydrolase contaminant present in Limulus coagulase sources interferes in, i.e., inhibits, coagulase-based assays for endotoxin. The contaminant inhibitor enzyme may be removed from coagulase by physical separation or by selective denaturation, for example by adjusting the pH of a coagulase solution to about from 8.5 to 11. Inhibitor-free coagulase is a novel composition. The coagulase activity of the composition is adjusted to a predetermined level to provide a standardized coagulase reagent for use in endotoxin assays.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.